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مقاله
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Abstract
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Title:
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The Effect Induced by miR-183 Cluster Overexpression on Transdifferentiation of Human Retinal Pigment Epithelial (RPE) Cells in Culture
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Author(s):
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Maliheh Davari, Zahra-Soheila Soheili, Shahram Samiei, Ehsan Ranaei Pirmardan
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Presentation Type:
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Oral
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Subject:
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Retina and Retinal Cell Biology
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Others:
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Presenting Author:
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Name:
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Maliheh Davari
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Affiliation :(optional)
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National Institute of Genetic Engineering and Biotechnology
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E mail:
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Maliheh_Davari@yahoo.com
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Phone:
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Mobile:
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09128152826
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Purpose:
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miR-183/96/182 cluster that highly expressed in the adult retina particularly in photoreceptors involves in development, maturation and normal function of neuroretina. It seems that overexpression of miR-183/96/182 cluster could potentially pave trans-differentiation of RPE cells into retinal neuron-like cells.
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Methods:
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RPE cells were transfected with the construct carrying miR-183/96/182. After 48 hours, total RNA was isolated, cDNAs were synthesized and Real-Time PCR was done to measure the expression level of miR-183/96/182 and that of some retinal specific neural genes such as Otx2, Nrl, Pdc, Dct, Pou4f2, Rcv.
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Results:
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Our data showed that all three miR-183, miR-96 and miR-182 were quantitatively overexpressed in the transfected RPE cells (100-, 240- and 56-fold, respectively). Furthermore, qPCR results implicated that the expression of neural genes including Otx2, Pdc, Dct, Pou4f2, Rcv was upregulated (3-, 7-, 2.5-, 2-, 4.7-fold, respectively) in RPE cells overexpressing miR-183 gene family.
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Conclusion:
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Upregulation of neural genes in miR-183/96/182-expressing RPE cells in comparison to control RPE cells suggests that in vitro overexpression of miR-183/96/182 could trigger RPE trans-differentiation into retinal neurons.
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Attachment:
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