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مقاله
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Abstract
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Title:
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Direct differentiation of Mesenchymal stem cells into retinal cells in vitro
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Author(s):
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Hamid Aboutalebkadkhodaeian1,TakiTiraihi,*1, Hamid Ahmadieh2, HoseinziaiiArdakani2, NarsisDaftarian2
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Presentation Type:
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Oral
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Subject:
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Retina and Retinal Cell Biology
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Others:
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Presenting Author:
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Name:
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Hamid Aboutaleb Kadkhodaeean
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Affiliation :(optional)
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1Dept. of Anatomical Sciences, School of Medical Sciences, TarbiatModares University, P.O. Box 14155-4838, Tehran, Iran. 2Department of Ophthalmology Labbafinejad Medical Center Pasdaran Ave. Boostan 9
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E mail:
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habootaleb92@gmail.com
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Phone:
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Mobile:
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09128598943
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Purpose:
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A hereditary degenerative disease of the retina, retinitis pigmentosa (RP), is the leading cause of visual handicap among working populations in developed countries, with an estimated 1.5 million patients worldwide. Here we used simple method for production of photoreceptor cells in vitro
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Methods:
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marrow stromal stem cells derived from male pigmented rats. The cells differentiated under the specific medium. After 14 days in vitro the specific markers for sensory retinal cells investigated to determine the differentiation of BMSCs to retinal cells.
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Results:
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Result showed that after 14 days in vitro, BMSCs cells differentiated into cells similar to sensory retina in morphology manner. The specific markers for each cell (OTX2 for progenitor cells), (Rhodopsin for rod photoreceptor), (NF200 for ganglionic cells), (GFAP for Müller glial cells), (Calretinin for Horizontal and Amacrin cells) revealed differentiation of BMSCs to retinal cells in vitro.
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Conclusion:
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For the first time in our study, we could differentiate the BMSCs cells to retinal cells such as photoreceptor, amacrine, ganglionic and muller glial cell in a simple method.
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Attachment:
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