Glaucoma is the second cause of blindness worldwide which intra-ocular pressure (IOP) within the eye rises. It is assumed that preexisting age-related changes of the trabecular meshwork (TM) play a role for the development of increased outflow resistance and IOP in various types of glaucoma. Today, after recognition of specific miRNA in eyes, the roles of these RNAs in development of eye and ocular disorders have been considered. This is a new insight into the molecular mechanisms of eye diseases because; the most targets of miRNAs have not been identified. LTBP2 is the second gene implicated in PCG after CYP1B1. LTBP2, encoded by LTBP2, is the largest member of the latent transforming growth factor (TGF)-beta binding protein family, that is localized in the anterior segment of the eye at the ciliary body. LTBP2 expression was shown in human eyes, including the trabecular meshwork which is considered to be affected in PCG. LTBP2 was shown to be essential for normal development of the anterior chamber of the eye, where it may have a structural role in maintaining ciliary muscle tone and its surrounding structures. In this study, we aimed to reveal how LTBP2 expression is affected by miRNA-204.

3'UTR of LTBP2 gene was cloned in an appropriated vector, the constructs transfected to eukaryotic cell, their expression were assayed in presence of miRNA-204 with Luciferase Assay, Real Time PCR and Western Bloting.

Our results suggest miRNA-204 (is expressed abundantly in eye) influence LTBP2 gene.

mir-204 in response to chronic oxidative stress and cellular senescence lead to pathogenic changes in the outflow pathway by increasing cell contraction, extracellular matrix deposition, and chronic production of reactive oxygen species and inflammatory mediators in the cells of the trabecular meshwork. LTBP2 is the largest member of the latent transforming growth factor (TGF)-beta binding protein family, which are extracellular matrix proteins with multidomain structure. It has homology to fibrillins and may have roles in cell adhesion and as a structural component of microfibrils. our finding reveal that mir-204 influence LTBP2 gene.